IKKβ antibody | knockout validation | Cell Signaling 8943

This is a knockout-validated antibody summary, based on the publication "The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

IKKβ antibody | knockout validation | Cell Signaling 8943 figure 1
Figure 1. Western blot analysis of cytoplasmic extracts from wild type (WT) 786-O cells and their knockout counterparts treated with 10 μM of sunitinib (Sun). From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: IKKβ

Catalog number: 8943

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human IKKβ protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Wild type and IKKβ-KO 786-O cells.

Blocking agent

5% nonfat dry milk overnight.

Primary incubation

For 2 h.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibody for 30 min.

Detection

Enhanced chemiluminescence (ECL Western Blotting Kit, Amersham).

References
  1. Makhov P, Naito S, Haifler M, Kutikov A, Boumber Y, Uzzo R, et al. The convergent roles of NF-κB and ER stress in sunitinib-mediated expression of pro-tumorigenic cytokines and refractory phenotype in renal cell carcinoma.. Cell Death Dis. 2018;9:374 pubmed publisher