This is a knockout-validated antibody summary, based on publications "Igf1R/InsR Function Is Required for Axon Extension and Corpus Callosum Formation", as cited below [1], "Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer" for western blot knockout validation (figure 4i, s5n) [2], and "Essential roles of insulin and IGF-1 receptors during embryonic lineage development" for western blot knockout validation (figure 1a) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. The expression of InsR and Igf1R in the cortex of heterozygous (+/-, Igf1r/Insr+/-: Insrflox/+;Igf1rflox/+;Emx1-CreC/+) and homozygous (-/-, Igf1r/Insr-/-: Insrflox/flox;Igf1rflox/flox;Emx1-CreC/+) E17 knockout embryos was analyzed by Western blot. Residual signals result from non-neural cells that are not affected by the cortex-specific knockout mediated by Emx1-Cre. The loading of comparable amounts of protein was verified using an anti-GAPDH antibody. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: IGF-I Receptor β

Catalog number: 9750

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human IGF-I receptor β protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

Control and (Igf1r-/-/Insr-/-) brain cortex-specific knockout mice. The cortex was dissected from the brains of embryonic day 17 (E17) embryos and lysed in ice cold RIPA buffer (1% NP40, 1% sodium deoxycholate, 0.1% SDS, 50mM HEPES (pH 7.4), 150mM NaCl, 10% Glycerol, 1.5mM MgCl2) for 1 h at 4˚C.

5% BSA in Tris-buffered saline (pH 7.4), 0.1% Tween-20) at 4˚C.

1:1,000 dilution.

1:3,000 dilution HRP-coupled anti-rabbit (115035033, Dianova).

Enhanced chemiluminescence detection system (Uptima, Interchim UP99619A).