This is a knockout-validated antibody summary, based on the publication "Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of IFT80 expression in IFT80f/f and IFT80d/d OB precursor cells. IFT80 protein level is normalized to GAPDH (n=3). From [1].

Rabbit polyclonal

Company: Abnova

Antibody: IFT80

Catalog number: PAB15842

Summary: Rabbit polyclonal antibody raised against partial recombinant protein corresponding to 134 amino acids of mouse Ift80. Reacts with mouse protein. Suitable for western blot.

Western blot

OB precursor cells obtained from calvarial bone from OSX;IFT80+/+ mice and OSX;IFT80f/f mice. Sample was homogenized with RIPA buffer (50 mM Tris, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, and 1% sodium deoxycholate) containing protease inhibitor cocktail (Sigma)

5% milk

1:400 overnight at 4 °C

horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:10,000, A-11034, Novex, Carlsbad, CA) at room temperature for 1 h

WesternBright ECL HRP (Advansta)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.