IFT20 antibody | knockout validation | Proteintech 13615-1-AP
DOI
//dx.doi.org/10.13070/ko.en.6.1857
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

IFT20 antibody | knockout validation | Proteintech 13615-1-AP figure 1
Figure 1. From figure 2 in [1]. Western blot of osteoblast lysates from wild-type and ITF20 knockout mice. Blot probed with antu-ITF20 antibody. Tubulin acts as a loading control.
Antibody information

Rabbit polyclonal

Company: Proteintech

Antibody: IFT20

Catalog number: 13615-1-AP

Summary: Rabbit polyclonal antibody raised against a human IFT20 fusion protein. Antigen affinity-purified. Supplier recommended for WB, IP, IHC, IF and elisa. Reacts with human, mouse, rat and Zebrafish IFT20.

Validation Method

Western blot

Sample

Osteoblast lysates from wild-type and IFT20 knockout mice.

Primary incubation

Anti-IFT20 antibody.

Detection

ECL reagent with Image Lab 5.0 (BioRad) for image analysis.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Noda K, Kitami M, Kitami K, Kaku M, Komatsu Y. Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development. Proc Natl Acad Sci U S A. 2016;113:E2589-97 pubmed publisher