This is a knockout-validated antibody summary, based on the publication "Regional astrocyte IFN signaling restricts pathogenesis during neurotropic viral infection", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1, κ

Company: BioLegend

Antibody: IFNAR-1

Catalog number: 127305

Summary: Mouse monoclonal IgG1, κ against the extracellular domain of murine IFNAR1. Reacts with mouse western blot, immunoprecipitation, flow cytometry and ELISA.

Immunohistochemistry

Cerebral cortex and cerebellum Ifnar^fl/fl and Ifnar^fl/fl Gfap-Cre⁺ mice. CNS tissue sections were obtained after cardiac perfusion with 4% paraformaldehyde in PBS.

Appropriate Alexa Fluor 555 antibodies (Life Technologies, Thermo Fisher Scientific).

Confocal microscopy (Carl Zeiss).

IHC detection of astrocyte marker ALDH1L1 (green) and IFNAR (red) in the cerebral cortex and cerebellum of uninfected Ifnar^fl/fl and Ifnar^fl/fl Gfap-Cre+ mice. Please see Figure S1a in the article [1].

The antibody is biotinylated to be used in FCM.

The same clone (MAR1-5A3) is sold as BioLegend 127301, 127302, 127303, 127304, 127305, 127306, 127311, 127312, 127313, 127314, 127321, 127322, 127323, 127324; Invitrogen 16-5945-85, 12-5945-80, 12-5945-82, 16-5945-38; Bio X Cell BE0241-5MG, BP0241-5MG; Bon Opus Biosciences BA020017; BD Biosciences 561183; GeneTex GTX14637.