This is a knockout-validated antibody summary, based on the publication "mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. SDS-PAGE and western blot analysis of whole cell lysates derived from HeLa (WT and IFITM3 KO). Immunoblotting was performed with anti-IFITM3 and anti-IFITM2/3. Actin served as loading control. From [1].

Mouse monoclonal IgG1

Company: Proteintech

Antibody: IFITM2/3

Catalog number: 66081-1-Ig

Summary: Mouse monoclonal IgG1 against IFITM2/3 fusion protein. Reacts with human by western blot, immunofluorescence, immunohistochemistry, flow cytometry and ELISA.

Western blot

The antibody detects 2 isoforms of the protein, and the KO is only for 1 of them.

WT and IFITM3 KO HeLa cells. Whole cell lysis was performed with radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher) supplemented with Halt Protease Inhibitor mixture EDTA-free (Thermo Fisher).

Odyssey blocking buffer in PBS (Li-Cor).

Secondary antibodies conjugated to DyLight 800 or 680 (Li-Cor).

Li-Cor Odyssey imaging system.