This is a knockout-validated antibody summary, based on the publication "Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: Mouse monoclonal IgG1κ

Catalog number: MAB2166

Summary: Mouse monoclonal IgG1κ against a fusion protein fragment of huntingtin (a.a. 181-810). Reacts with human, mouse, rat, monkey, hamster, and rabbit. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry, immunoprecipitation and ELISA.

Western blot

Different brain region samples were homogenized in the Nonidet P-40 buffer (50 mM Tris pH 7.4, 50 mM NaCl, 0.1% Triton X-100, 1% Nonidet P-40) or RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, pH 8.0, 0.1% SDS, 0.5% DOC, and 1% Triton X-100) with 1× protease inhibitor (Sigma, P8340)

SuperSignal West Femto Maximum Sensitivity Substrate (34095) or Amersham ECL Prime Western Blotting Detection Reagent (RPN2232).

Western blots showing relative Htt protein levels in the brain of ubiquitous KO and control mice at 2, 4, and 8 mo of age. Ctx, cortex; cere, cerebellum; bs, brainstem; str, striatum; oth, other brain regions. Please see Figure 1b in the article [1].

The same clone (1HU-4C8) is sold as Invitrogen MA3-040; EMD Millipore MAB2166.