This is a knockout-validated antibody summary, based on the publication "Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: Huntingtin

Catalog number: ab109115

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human Huntingtin aa 1-100. Reacts with mouse, rat, and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

Brain tissues from control and ubiquitous and neuronal Htt-KO mice. Protein samples were homogenized in the Nonidet P-40 buffer (50 mM Tris pH 7.4, 50 mM NaCl, 0.1% Triton X-100, 1% Nonidet P-40) or RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, pH 8.0, 0.1% SDS, 0.5% DOC, and 1% Triton X-100) with 1× protease inhibitor (Sigma, P8340).

SuperSignal West Femto Maximum Sensitivity Substrate (34095) or Amersham ECL Prime Western Blotting Detection Reagent (RPN2232).

The same clone (EPR5526) is sold as Abcam ab109115; EMD Millipore MABN1105.