This is a knockout-validated antibody summary, based on the publication "Prevention of acquired sensorineural hearing loss in mice by in vivo Htra2 gene editing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. The western blot results of protein disruption after transfection with Sp-g1, Sp-g2, and Sp-g3, respectively, in HEI-OC1 cells. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: HtrA2/Omi

Catalog number: 9745

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Phe341 of human HtrA2/Omi protein. Reacts with human, mouse, rat and monkey. Suitable for western blot.

Western blot

Control and HtrA2/Omi knockout HEI-OC1 cells.

5% non-fat dry milk (Cell Signaling Technology, #9999S).

1:1,000 dilution at 4°C overnight

1:5,000 dilution HRP-conjugated secondary antibodies for 1h at room temperature.

Meilunbio® fg super sensitive ECL luminescence reagent (Meilunbio, #MA0186).