This is a knockout-validated antibody summary, based on the publication " Therapeutic inducers of the HSP70/HSP110 protect mice against traumatic brain injury", as cited below [1] and "Genomic innovation of ATD alleviates mistranslation associated with multicellularity in Animalia" for western blot knockout validation (see [2] figure 1b). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 2 in [1]. Western blot of cortex lysates from wild-type and Hsp70 knockout mice probed with anti-Hsp170 antibody. Actin serves as a loading control.

Mouse monoclonal

Company: StressMarq Biosciences

Antibody: Hsp70

Catalog number: SMC-100

Summary: Mouse monoclonal antibody raised against human Hsp70.

Supplier recommended for WB, IHC, ICC and flow cytometry.

Reacts with human, dog, monkey, rat, Guinea pig, hamster, fish, Drosophila, nematode, chicken, pig, sheep, cow and rabbit Hsp70.

Western blot

Lysates of brain cortex from wild-type and Hsp70 knockout mice.

Anti-Hsp70 antibody at 1:1000 dilution.

NIH Image J 1.47v software.

The same clone (C92F3A-5) is sold as Novus Biologicals NB110-61582; OriGene TA309388, TA326356; Santa Cruz Biotechnology sc-66048; LifeSpan Biosciences LS-C82983, LS-C108950; Enzo Life Sciences ADI-SPA-810-D, ADI-SPA-810-488-D, ADI-SPA-810AP-D, ADI-SPA-810B-D, ADI-SPA-810FI-D, ADI-SPA-810PE-D, ADI-SPA-810-J, ADI-SPA-810-F, ADI-SPA-810-488-F, ADI-SPA-810AP-F, ADI-SPA-810B-F, ADI-SPA-810FI-F, ADI-SPA-810PE-F; Abcam ab47455; Rockland Immunochemicals 200-301-A27; StressMarq Biosciences SMC-100B; Invitrogen MA1-10889.