Hsp70-FITC antibody | knockout validation | multimmune cmHsp70.1
DOI
//dx.doi.org/10.13070/ko.en.7.2219
Date
2017-03-18

This is a knockout-validated antibody summary, based on the publication "Tumor imaging and targeting potential of an Hsp70-derived 14-mer peptide", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Hsp70-FITC antibody | knockout validation | multimmune cmHsp70.1 figure 1
Figure 1. Immunofluorescence staining of Hsp70 (green staining) in viable 4T1 wt and Hsp70−/− cells. Cells were counter-stained with DAPI (blue staining). From [1].
Antibody information

Mouse monoclonal IgG1

Company: multimmune GmbH

Antibody: Hsp70-FITC

Catalog number: cmHsp70.1

Summary: Mouse monoclonal IgG1 against cell surface localized human Hsp70. Reacts with human and mouse. Suitable for western blot, immunocytochemistry, immunohistochemistry and flow cytometry.

Validation Method

Immunocytochemistry

Sample

Wild type and Hsp70−/− 4T1 mouse breast cancer cell lines.

Secondary incubation

Appropriate Cy3-conjugated anti-goat and anti-rabbit immunoglobulin secondary antibodies were obtained from Jackson ImmunoResearch (Newmarket, UK).

References
  1. Gehrmann M, Stangl S, Foulds G, Oellinger R, Breuninger S, Rad R, et al. Tumor imaging and targeting potential of an Hsp70-derived 14-mer peptide. PLoS ONE. 2014;9:e105344 pubmed publisher