Hsp27 antibody | knockout validation | Santa Cruz sc-13132
DOI
//dx.doi.org/10.13070/ko.en.7.2140
Date
2017-02-17

This is a knockout-validated antibody summary, based on the publication "The Invalidation of HspB1 Gene in Mouse Alters the Ultrastructural Phenotype of Muscles", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Hsp27 antibody | knockout validation | Santa Cruz sc-13132 figure 1
Figure 1. Western blot of Hsp27 in muscle from HspB1-/- and wild-type (HspB1+/+) mouse. From [1].
Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Hsp27

Catalog number: sc-13132

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 32-108 of human HSP27. Recommended for detection of HSP27 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Muscle of HspB1-/- and wild-type (HspB1+/+) mouse.

Blocking agent

TBS 1x buffer containing 5% skimmed milk.

Primary incubation

1/1,000 dilution overnight at room temperature.

Secondary incubation

Fluorochrome-conjugated LICOR-antibody at 37°C for 30 min.

Detection

Scanner Odyssey (LI-COR Biosciences) at 800 nm.

References
  1. Kammoun M, Picard B, Astruc T, Gagaoua M, Aubert D, Bonnet M, et al. The Invalidation of HspB1 Gene in Mouse Alters the Ultrastructural Phenotype of Muscles. PLoS ONE. 2016;11:e0158644 pubmed publisher