This is a knockout-validated antibody summary, based on the publication "A network-based approach for predicting Hsp27 knock-out targets in mouse skeletal muscles", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Hsp27 protein abundance ( in arbitrary units) in the muscle Tibialis Anterior and Soleus of mice. From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz Biotechnology

Antibody: Hsp27

Catalog number: sc-13132

Summary: Mouse monoclonal IgG2a (kappa light chain) raised against amino acids 32-108 of HSP 27 of human origin. Recommended for detection of HSP 27 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

muscles of HspB1-null mice and controls

5% non-fat milk in TBS1 x buffer

1/1000 overnight at room temperature

fluorochrome-conjugated LICOR-antibody at 37°C for 30 minutes

Infrared fluorescence detection with Odyssey scanner (LI-COR Biosciences) at 800 nm

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.