This is a knockout-validated antibody summary, based on the publication "Defective heat shock factor 1 inhibits the growth of fibrosarcoma derived from simian virus 40/T antigen‑transformed MEF cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Hsf1 in the SV40/TAG-transformed MEF cell lines: Lane 1, MEF/wt; lane 2, MEF/Hsf1-/-; and lane 3, MEF/mHsf1. From [1].

Antibody: Hsf1

Catalog number: 4356

Summary: Rabbit polyclonal raised against a synthetic peptide corresponding to amino acids at the carboxy-terminus of human HSF1 protein. Reacts with human, mouse, rat, monkey and has been used in western blotting, immunoprecipitation, immunohistochemistry (paraffin), immunocytochemistry, flow cytometry and chromatin IP.

Company: Cell Signaling

Western blot

Hsf1 null MEFs, lysed in NP-lysis buffer (50 mM Tris-HCl, pH 7.4; 150 mM NaCl, 1% NP-40 and 1X cocktail protease inhibitor (Sigma-Aldrich).

5% non-fat dried milk/Tris-buffered saline-Tween 20.

1:1,000, at 4C overnight.

horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature.

enhanced chemiluminescence and exposed to X-ray film (Thermo Fisher Scientific).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.