This is a knockout-validated antibody summary, based on the publication "IRE1α is an endogenous substrate of endoplasmic-reticulum-associated degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal
Company: Novus Biologicals
Antibody: Hrd1
Catalog number: NB100-2526
Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to a region within residues 300-400 of human Hrd1.
Supplier recommended for WB, IHC and ICC.
Reacts with human and mouse Hrd1.
Western blot
Lysates of wild-type and Hrd1 knockout mouse embryonic fibroblasts.
Anti-Hrd1 diluted 1:8000.
Image Lab ChemiDOC XRS+ (BioRad).
See Figure 1 in [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
- product