HoxB13 antibody | knockout validation | Santa Cruz sc-28333

This is a knockout-validated antibody summary, based on the publication "MEIS-mediated suppression of human prostate cancer growth and metastasis through HOXB13-dependent regulation of proteoglycans", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HoxB13 antibody | knockout validation | Santa Cruz sc-28333 figure 1
Figure 1. Western blot analysis of HOXB13 knockout using CRISPR and exogenous expression of MEIS1 in the resulting HOXB13ko cells for CWR22Rv1 and LAPC4. Actin was used as a loading control. From [1].
Antibody information

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: HoxB13

Catalog number: sc-28333

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1-284 of human HoxB13. Recommended for detection of HoxB13 of human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Control and HOXB13-KO CWR22Rv1 and LAPC4 cell lines.

Blocking agent

TBS+5% nonfat milk for 1 hr at room temperature.

Primary incubation

1:100 dilution in TBST+5% nonfat milk overnight at 4°C.

Secondary incubation

1:10,000 dilution in TBST+5% nonfat milk for 1 hr at room temperature.

Detection

Odyssey imaging system (LI-COR Biosciences) and analyzed with LI-COR Image Studio software.

References
  1. VanOpstall C, Perike S, Brechka H, Gillard M, Lamperis S, Zhu B, et al. MEIS-mediated suppression of human prostate cancer growth and metastasis through HOXB13-dependent regulation of proteoglycans. elife. 2020;9: pubmed publisher