Histone Deacetylase 5 (HDAC5) antibody | knockout validation | Sigma H4538

This is a knockout-validated antibody summary, based on the publication "Class IIa HDACs regulate learning and memory through dynamic experience-dependent repression of transcription", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Histone Deacetylase 5 (HDAC5) antibody | knockout validation | Sigma H4538 figure 1
Figure 1. Total protein homogenates from cortices and cerebella of adult wildtype (Control) and Hdac4flox/flox/Hdac5-/-/Emx1IRES-Cre (DKO) mice were probed by immunoblotting with antibodies against HDAC4, HDAC5 and bTubulin (as a loading control). Please see Figure S5b in the article [1].
Antibody information

Mouse monoclonal IgG1

Company: Sigma

Antibody: Histone Deacetylase 5 (HDAC5)

Catalog number: H4538

Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to residues in human histone deacetylase 5 (HDAC5). Reacts with mouse, rat and human. Suitable for western blot, immunocytochemistry, immunoprecipitation, ELISA and microarray.

Validation Method

Western blot

Sample

Control and HDAC5-KO mouse brain cortex and cerebellum.

Clone note

The same clone (HDAC5-35) is sold as Abcam ab50001; MilliporeSigma H4538.

References
  1. Zhu Y, Huang M, Bushong E, Phan S, Uytiepo M, Beutter E, et al. Class IIa HDACs regulate learning and memory through dynamic experience-dependent repression of transcription. Nat Commun. 2019;10:3469 pubmed publisher