This is a knockout-validated antibody summary, based on the publication "Hepatitis B virus rigs the cellular metabolome to avoid innate immune recognition", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. HK2 WT (WT) and HK2−/− cells were transfected with control vector or pHBV-1.3 (genotype D) for 36 h and transfected with or without poly(I:C) (1 μg/mL) for 12 h. Co-IP and immunoblot analysis were performed with the indicated antibodies. From [1].

Mouse monoclonal IgG1

Company: Abcam

Antibody: Hexokinase II

Catalog number: ab104836

Summary: Mouse monoclonal antibody against a recombinant fragment corresponding to human Hexokinase II. Reacts with human by western blot, immunohistochemistry (paraffin) and flow cytometry.

Western blot

WT and HK2−/− HepG2 cells.

5% (w/v) non-fat milk.

1:500 dilution at 4 °C overnight.

Horseradish peroxidase-linked secondary antibodies (Jackson ImmunoResearch) for 1 h.

The signal of immunoreactive bands was visualized using a LAS-4000 image document instrument (FujiFilm, Tokyo, Japan).

The same clone (3D3) is sold as Novus Biologicals NBP1-51643; OriGene AM06497SU-N; LifeSpan Biosciences LS-B8935; Abnova MAB10684, MAB16326; Abcam ab104836; Invitrogen MA5-15679; ProMab 30088.