Hexokinase I antibody | knockout validation | Cell Signaling 2024

This is a knockout-validated antibody summary, based on the publication "KRAS4A directly regulates hexokinase 1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Hexokinase I

Catalog number: 2024

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human hexokinase I. Reacts with human and mouse. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

WT and HK1-deficient HEK293 cells.

Primary incubation

1:2,000 dilution.

Figure

WT (n=6) or HK1- or HK2-deficient (n=4) HEK293 cells were transfected with FLAG-tagged GFP, KRAS4B12V, or KRAS4A12V, and 24-h glucose consumption (mean±SE) was determined. Immunoblot shows relative expression. Please see Figure 4d in the article [1].

References
  1. Amendola C, Mahaffey J, Parker S, Ahearn I, Chen W, Zhou M, et al. KRAS4A directly regulates hexokinase 1. Nature. 2019;576:482-486 pubmed publisher