Heterochromatin Protein-1 gamma antibody | knockout validation | EMD Millipore MAB3450
DOI
//dx.doi.org/10.13070/ko.en.7.2251
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Heterochromatin Protein-1 gamma antibody | knockout validation | EMD Millipore MAB3450 figure 1
Figure 1. Western blotting for HP1γ on nuclear extracts from WT and HP1γ-null (clone H5) ES23+ cells. Histone H3 is shown as a control. From [1].
Antibody information

Mouse monoclonal IgG

Company: EMD Millipore

Antibody: Heterochromatin Protein-1 gamma

Catalog number: MAB3450. The product (MAB3450) appears no longer available from EMD Millipore. The same clone (2MOD-1G6) is available from ThermoFisher Invitrogen as MA3-054 or Active Motif 39981.

Summary: Mouse monoclonal IgG1 against recombinant mouse HP1-gamma. Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry, immunocytochemistry/immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and HP1γ-null ES23+ cells.

Blocking agent

5% milk in Tris-buffered saline (TBS) plus 0.1% Tween 20 (TBST).

Primary incubation

Diluted in 2.5% milk in TBST.

Secondary incubation

Diluted in 2.5% milk in TBST.

Clone note

The same clone (2MOD-1G6) is sold as Active Motif 39981; Invitrogen MA3-054.

References
  1. Brideau N, Coker H, Gendrel A, Siebert C, Bezstarosti K, Demmers J, et al. Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome. Mol Cell Biol. 2015;35:4053-68 pubmed publisher