Hdac2 antibody | knockout validation | Millipore 05-814
DOI
//dx.doi.org/10.13070/ko.en.7.2127
Date
2017-02-15

This is a knockout-validated antibody summary, based on the publication "Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG1

Company: Millipore

Antibody: Hdac1

Catalog number: 05-814

Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to amino acids 473-488 of human HDAC2. Reacts with human, bovine, mouse, rat and hamster. Suitable for western blot, immunoprecipitation and immunoprecipitation.

Validation Method

Western blot

Sample

Hdac1/2-KO E14 ES cell line after the induction of gene deletion (0–4 d).. Whole-cell extracts were prepared in IP buffer (20 mM Hepes, pH 7.4, 250 mM NaCl, 0.5% IGEPAL CA-630, and 1× protease inhibitor mix (Sigma)) at 4 °C for 30 min.

Primary incubation

1:2,000 dilution.

Secondary incubation

IRDye-conjugated secondary antibodies (LI-COR Biosciences).

Detection

Odyssey Infrared Imaging System.

Figure

Please see Figure 1b in the article [1].

Clone note

The same clone (3F3) is sold as Santa Cruz Biotechnology sc-81599; ImmuQuest IQ544, IQ544FITC, IQ544HRP; Invitrogen MA5-18061; EMD Millipore 05-814.

References
  1. Jamaladdin S, Kelly R, O'regan L, Dovey O, Hodson G, Millard C, et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A. 2014;111:9840-5 pubmed publisher