This is a knockout-validated antibody summary, based on the publication "HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: HSF1
Catalog number: 12972
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the carboxy terminus of human HSF1 protein. Reacts with human, mouse, rat, monkey, bovine, dog, and pig. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry, flow cytometry and ChIP.
Western blot
WT and HSF1-KO HeLa cells. Cells were extracted with ice-cold PBS containing 0.5% Triton X-100 (Wako Pure Chemical Industries, 168-11805) and a protease inhibitor cocktail (Nacalai Tesque, 25955-11).
10% skimmed milk, 5% bovine serum albumin (Nacalai Tesque, 01860-36), or Blocking Solution (Nacalai Tesque, 05151-35) for 1 h.
For 12 h at 25°C.
1:4,000 dilution alkaline phosphatase-conjugated anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc., 111-055-144).
Nitroblue tetrazolium chloride (Nacalai Tesque, 24720-01) and 5-bromo-4-chloro-3-indolylphosphate P-toluidine salt (Nacalai Tesque, 05643-11) reagents.
Cell lysates of WT and individual HSF1-KO HeLa cell clones (#6 and #12) were subjected to immunoblot analysis with anti-HSF1 and anti-actin. From [1].
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