This is a knockout-validated antibody summary, based on the publication "Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: HPPD

Catalog number: sc-271672

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 1-300 mapping at the N-terminus of human HPPD. Recommended for detection of HPPD of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Immunohistochemistry

Liver sections from control and HPD-KO. Paraffin sections were treated with Antigen Retrieval Solution (Dako, Copenhagen, Denmark, catalog no. S236984) for 30 minutes.

3% hydrogen peroxide for 5 minutes.

1:100 dilution at 4°C overnight.

M.O.M Vectastain and DAB detection kit (from Vector Laboratories, Burlingame, CA, catalog no. PK-2200 and SK-4100, respectively) according to the manufacturer’s recommendations.

Amersham ECL Western Blotting Detection Reagent (General Electric Healthcare Life Sciences, Marlborough, MA, catalog no. RPN2106).

Immunostaining for HPD in the liver 1 year after CRISPR/Cas9 editing. HPD-positive hepatocytes (Fah −/−/Hpd +/+) are stained in brown. Liver sections of mice 1 year after injection show that hepatocytes lacking HPD (clear) have completely repopulated the liver. Arrow points toward one of the few HPD-positive hepatocytes. Boxed areas are shown enlarged to the right. Scale bar represents 50 μm. Please see Supplementary Figure 1 in the article [1].