HPGDS antibody |knockout validation | MyBiosource MBS601438

DOI

http://dx.doi.org/10.13070/ko.en.6.1608

Date

2016-09-09

This is a knockout-validated antibody summary, based on the publication "Oviductal estrogen receptor α signaling prevents protease-mediated embryo death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HPGDS antibody |knockout validation | MyBiosource MBS601438 figure 1

Figure 1. Immunoblot of HPGDS expression in WT and cKO oviducts at 0.5 days post coitum; β-actin was used as a loading control. From figure 4 [1].

Antibody information

Company: MyBiosource

Antibody: HPGDS

Catalog number: MBS601438

Summary: Rat IgG raised against recombinant mouse hematopoietic prostaglandin D synthase (H-PGDS). Recognizes mouse and human H-PGDS.. Suitable for use in immunohistochemistry and western blot.

Validation Method

Western blot, WB

Sample

Immunoblot of HPGDS expression in WT and cKO oviducts at 0.5 dpc; β-actin was used as a loading control. Protein lysate from one mouse in each lane; n = 4–5 mice/group. For oviduct immunoblots, protein was extracted and analyzed.

Primary incubation

antibodies were used at a 1:1000 dilution.

References

Winuthayanon W, Bernhardt M, Padilla-Banks E, Myers P, Edin M, Hewitt S, et al. Oviductal estrogen receptor α signaling prevents protease-mediated embryo death. elife. 2015;4:e10453 pubmed publisher

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