HMGB1antibody | knockout validation | Abcam ab79823

This is a knockout-validated antibody summary, based on the publication "HMGB1 orchestrates STING-mediated senescence via TRIM30α modulation in cancer cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HMGB1antibody | knockout validation | Abcam ab79823 figure 1
Figure 1. Immunoblot of the indicated proteins in HMGB1-WT and -KO MEFs treated with 100 ng/ml Dox for 3 days. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: HMGB1

Catalog number: ab79823

Summary: Rabbit monoclonal antibody against a synthetic peptide of HMGB1. Reacts with Mouse, Rat, Human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation.

Validation Method

Western blot

Sample

WT and HMGB1-KO MEFs.

Blocking agent

5% (w/v) nonfat dry milk in 1× phosphate-buffered saline (PBS) overnight at 4 °C.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibodies.

Detection

Standard chemiluminescence method performed according to the manufacturer’s instructions.

References
  1. Lee J, Park I, Kwak M, Rhee W, Kim S, Shin J. HMGB1 orchestrates STING-mediated senescence via TRIM30α modulation in cancer cells. Cell Death Discov. 2021;7:28 pubmed publisher