HINT1 antibody | knockout validation | Abcam ab124912

This is a knockout-validated antibody summary, based on the publication "HINT1 (Histidine Triad Nucleotide-Binding Protein 1) Attenuates Cardiac Hypertrophy Via Suppressing HOXA5 (Homeobox A5) Expression", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HINT1 antibody | knockout validation | Abcam ab124912 figure 1
Figure 1. Western blotting of HOXA5 expression in heart samples from WT or HINT1 KO mice subjected to sham or TAC surgery for 4 weeks. n=6 mice per group. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: HINT1

Catalog number: ab124912

Summary: Rabbit monoclonal antibody against a synthetic peptide within human HINT1. Reacts with human. It is Predicted to work with mouse and rat. Suitable for western blot, immunohistochemistry (paraffin) and immunocytochemistry/immunofluorescence.

Validation Method

Western blot

Sample

WT and HINT1 KO mouse heart tissues.

Secondary incubation

Anti-rabbit HRP-conjugated antibodies.

Detection

Chemiluminescence was generated and detected by ChemiDoc+(Bio-RAD).

References
  1. Zhang Y, Da Q, Cao S, Yan K, Shi Z, Miao Q, et al. HINT1 (Histidine Triad Nucleotide-Binding Protein 1) Attenuates Cardiac Hypertrophy Via Suppressing HOXA5 (Homeobox A5) Expression. Circulation. 2021;144:638-654 pubmed publisher