HIF-2alpha antibody | knockout validation | Novus Biologicals NB100-122
DOI
//dx.doi.org/10.13070/ko.en.6.1638
Date
2016-09-24

This is a knockout-validated antibody summary, based on the publication "Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HIF-2alpha antibody | knockout validation | Novus Biologicals NB100-122 figure 1
Figure 1. Immunoblot analyses of ear mesenchymal stem cells (EMSCs) derived from control and HIF-2alpha KO mice treated with 0.1 mM dimethyloxalylglycine (DMOG) for 24 hrs, from figure 1 [1].
Antibody information

Antibody: HIF-2alpha

Catalog number: NB100-122

Summary: Rabbit polyclonal raised against a peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. Detects human, mouse, rat, fish, hamster, primate, sheep. And has been used in western blot, simple western, ChIP, ELISA, flow cytometry, Gel Super Shift assays, immunocytochemistry, immunohistochemistry, and immunoprecipitation.

Company: Novus Biologicals

Validation Method

Western blot, WB

Sample

Ear mesenchymal stem cells isolated from HIF2-alpha floxed mice. Nuclear proteins were isolated using the NE-PER nuclear extraction kit according to manufacturer’s protocol (Thermo Scientific)

References
  1. Mori H, Yao Y, Learman B, Kurozumi K, Ishida J, Ramakrishnan S, et al. Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion. Sci Rep. 2016;6:21520 pubmed publisher