This is a knockout-validated antibody summary, based on the publication "Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analyses of ear mesenchymal stem cells (EMSCs) derived from control and HIF-1alpha KO mice treated with 0.1 mM dimethyloxalylglycine (DMOG) for 24 hrs, from figure 1 [1].

Company: Novus Biologicals

Antibody: HIF-1alpha

Catalog number: NB100-105

Summary: Mouse monoclonal IgG2b raised against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha. Detects human, mouse, rat, porcine, bovine, ferret, monkey, primate, rabbit, sheep, Xenopus, yeast proteins and has been used in western blot, ChIP, ELISA, flow cytometry, Gel Super Shift assays, immunocytochemistry, immunohistochemistry, immunoprecipitation, tissue culture substratum, and ligand activation.

Catalog number NB100-134

Summary: Rabbit polyclonal raised against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha. Detects human, mouse, rat, canine, guinea pig, primate, Xenopus. It has been used in western blot, simple western, ChIP, Gel Super Shift assays, immunocytochemistry, immunohistochemistry, and immunoprecipitation.

WB, western blot

Ear mesenchymal stem cells isolated from HIF1-alpha floxed mice. Nuclear proteins were isolated using the NE-PER nuclear extraction kit according to manufacturer’s protocol (Thermo Scientific).