HGK antibody | knockout validation | Cell Signaling 5146

This is a knockout-validated antibody summary, based on the publication "HGK promotes metastatic dissemination in prostate cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

HGK antibody | knockout validation | Cell Signaling 5146 figure 1
Figure 1. Western-blot analysis of HGK normalized with β-actin in PC3 cells to confirm depletion. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: HGK

Catalog number: 5146

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Ala553 of human HGK protein. Reacts with human, mouse and monkey. Suitable for western blot.

Validation Method

Western blot

Sample

Control and HGK KO PC3 cells.

Blocking agent

5% not fat milk in Tween 20 Tris-buffered saline (TTBS) for 1 h.

Primary incubation

1:1,000 diluted in 5% BSA TTBS overnight.

Secondary incubation

1:5,000 dilution anti-rabbit IgG SCT, sc-2357 antibodies conjugated with HRP in 5% BSA TTBS for 1 h.

Detection

Clarity Western ECL Substrate (Bio-Rad) and detected with VWR image view station.

References
  1. Garcia Garcia S, Rodrigo Faus M, Fonseca N, Manzano S, Gyorffy B, Ocana A, et al. HGK promotes metastatic dissemination in prostate cancer. Sci Rep. 2021;11:12287 pubmed publisher