This is a knockout-validated antibody summary, based on the publication "Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of HFE2 in 3000g fraction from mouse liver homogenates (3000g), whole liver homogenates prepared in 1% NP40 (NP40) or microsomes (Micros). Samples were prepared from Hfe2+/+ mice (WT) and Hfe2-/- mice (KO). Arrows denote the HFE2-specific bands. In contrast to the NP40 whole liver homogenate or liver microsomes, HFE2 is present in the 3000 g fraction solely as a full-length protein. From [1].

Goat polyclonal IgG

Company: R&D Systems

Antibody: HFE2

Catalog number: AF3634

Summary: Goat polyclonal IgG against mouse myeloma cell line NS0-derived recombinant mouse RGM-C isoform 1 Gln33-Asp393 (Ile379Val). Reacts with mouse by western blot.

Western blot

Mouse liver preparations from WT and Hfe2-/- mice.

5 % milk in tris-buffered saline containing 0.1 % Tween 20 for 1h.

1:1,500 dilution in 5 % milk overnight.

1:40,000 dilution donkey anti-goat (705-036-147, Jackson Immunoresearch).