This is a knockout-validated antibody summary, based on the publication "Erb-b2 Receptor Tyrosine Kinase 2 (ERBB2) Promotes ATG12-Dependent Autophagy Contributing to Treatment Resistance of Breast Cancer Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: HER2/ErbB2
Catalog number: 2165
Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding tyrosine 1248 of human ErbB2 protein. Reacts with human and mouse. It is predicted to react based on 100% sequence homology with rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry (fixed/permeabilized).
Western blot
Control and ERBB2-KO SKBR3 cells.
Goat anti-rabbit IgG (H+L)–HRP conjugate (Bio-Rad Laboratories, 170–6515).
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.