This is a knockout-validated antibody summary, based on the publication "Deletion of histone deacetylase 7 in osteoclasts decreases bone mass in mice by interactions with MITF", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting of bone marrow macrophage cultures from Hdac7flox/Hdac7flox mice infected either with control adenovirus encoding EGFP (Ad-C) or encoding CRE recombinase (Ad-Cre). α- tubulin was used as loading control. From [1].

Rabbit polyclonal

Company: Abcam

Antibody: HDAC7

Catalog number: ab12174

Summary: Rabbit polyclonal to a synthetic peptide (KPRLRQIPSAEDLETDG) conjugated to KLH, corresponding to amino acids 436-452 of Human HDAC7 isoform a. Reacts with mouse, rat, human and is suitable for western blot, immunoprecipitation, and immunocytochemistry.

Western blot

bone marrow macrophage cultures from Hdac7flox/Hdac7flox mice

1:2000 dilution in TBS/0.1% Tween-20 plus 3% bovine serum albumin at 4°C

Horseradish-peroxidase conjugated anti-rabbit (NA-934, Amersham) at 1:8000 dilution, in TBS/0.1% Tween-20 plus 5% nonfat dry milk.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.