This is a knockout-validated antibody summary, based on the publication "Quantitative Temporal Proteomic Analysis of Vaccinia Virus Infection Reveals Regulation of Histone Deacetylases by an Interferon Antagonist", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot confirming knockout of HDAC5. Sequencing of genomic DNA from clones H5KO3 and H5KO4 confirmed frameshift mutations in both alleles. From [1].

Mouse monoclonal IgG2b, κ

Company: Santa Cruz

Antibody: HDAC5

Catalog number: sc-133225

Summary: Mouse monoclonal IgG2b, κ against amino acids 371-443 of human HDAC5. Recommended for detection of HDAC5 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

WT and HDAC5-KO HeLa cells.

5% non-fat milk in TBS containing 0.1% Tween-20 for 30 min at room temperature.

1:500 dilution in blocking solution.

1:10,000 dilution goat anti-mouse IgG IRDye 680LT-conjugated (926-68020) or IRDye 800CW-conjugated (926-32210, LI-COR Biosciences) in blocking solution.

The reactive bands were detected using an Odyssey infrared imager (LI-COR Biosciences).