This is a knockout-validated antibody summary, based on the publication "Hypothalamic leptin action is mediated by histone deacetylase 5", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative western blot of reference protein ACTIN and HDAC5 in primary hypothalamic neurons isolated from KO and WT mice that were subjected to saline or leptin (100 ng ml−1) treatment for 30 min (n=3). From [1].

Mouse monoclonal IgG2b

Company: Santa Cruz

Antibody: HDAC5

Catalog number: sc-133106

Summary: Mouse monoclonal IgG2b raised against amino acids 371-443 of HDAC5 of human origin. Recommended for detection of HDAC5 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

primary hypothalamic neurons isolated from HDAC5-KO and WT mice

1:2,000 dilution

conventional film detection, using ECL or prime ECL (Amersham Biosciences, Piscataway, NJ, USA)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.