This is a knockout-validated antibody summary, based on the publication "HDAC4 is required for inflammation-associated thermal hypersensitivity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blots HDAC4 in dorsal root ganglion of HDAC4fl/fl, Nav1.8 Cre, and Advillin-CreERT2 mouse lines. From [1].

Antibody: HDAC4

Catalog number: sc-11418

Summary: Rabbit polyclonal raised against epitope corresponding to amino acids 530-631 of HDAC4 of human origin. Recommended for detection of HDAC4 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Company: Santa Cruz Biotechnology

Western blot

Dorsal root ganglion of HDAC4fl/fl, Nav1.8 Cre, and Advillin-CreERT2 mouse lines. Protein was extracted in 0.2% SDS in double-distilled H2O with 1× protease inhibitor cocktail (Roche Diagnostics).

5% milk

overnight at 4C, 1:500

1:5000 horseradish peroxidase–conjugated anti-rabbit (GE Healthcare, Little Chalfont, United Kingdom) for 1h

Enhanced chemiluminescence prime kit (GE Healthcare) and visualized with a UVP GelDoc-It Imaging system (Ultra-Violet Products, Cambridge, United Kingdom).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.