This is a knockout-validated antibody summary, based on the publication "Histone deacetylase 3 associates with MeCP2 to regulate FOXO and social behavior", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a

Company: Cell Signaling

Antibody: HDAC3

Catalog number: 3949

Summary: Mouse monoclonal IgG2a against recombinant human HDAC3 protein. Reacts with human, mouse, rat, and monkey by western blot, immunoprecipitation, and immunofluorescence (immunocytochemistry).

Immunohistochemistry

Coronal brain slices of 3-month control and Hdac3 cKO male mice. Mice were perfused with 10% formaldehyde under deep anesthesia and brains were post-fixed overnight in 10% formaldehyde. Brains were sectioned at 40 μm using a vibratome (Leica).

PBS containing 0.3% Triton X-100 and 10% normal donkey serum at room temperature (20–22 °C) for 1 h.

1:200 dilution in blocking buffer overnight at 4 °C.

Alexa Fluor 488, Alexa Fluor 568, and Alexa Fluor 647 antibodies diluted 1:500 in PBS and incubated at 20–22 °C for 90 min.

Images were acquired using an LSM 710 Zeiss confocal microscope.

Please see Supplementary Figure 1a,c,d in the article [1].

The same clone (7G6C5) is sold as LifeSpan Biosciences LS-B3894; Abnova MAB12608; Invitrogen MA5-15442; Cell Signaling Technology 3949.