This is a knockout-validated antibody summary, based on the publication "MDM2 E3 ligase-mediated ubiquitination and degradation of HDAC1 in vascular calcification", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of vascular smooth muscle-specific HDAC1 deletion. Aorta samples were used for the detection of HDAC1 in either HDACfl/fl mice (WT) or SM22-cre;HDAC1fl/fl mice (HDAC1-cKO). From [1].

Mouse monoclonal IgG1

Company: Millipore

Antibody: HDAC1

Catalog number: 05-100

Summary: Mouse monoclonal IgG1 against a synthetic peptide corresponding to amino acids 467-482 of mouse HDAC1. Reacts with mouse and human. Suitable for western blot and immunoprecipitation.

Western blot

Aorta samples from control HDACfl/fl mice or vascular smooth muscle-specific SM22-cre;HDAC1fl/fl mice. Tissues were homogenized in lysis buffer ((50 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1% NP-40, 1 mM DTT, 1 mM phenylmethylsulfonyl fluoride, 1 mM Na3VO4 and 1 μg ml−1 each of leupeptin, pepstatin and aprotinin).

5% skim milk in 1 × TBST.

1:1,000 dilution overnight at 4 °C.

Horseradish peroxidase-linked secondary antibodies for 1 h at room temperature.

Enhanced chemiluminescence using a Fuji LAS-3000 system (Fujifilm Life Science, Tokyo, Japan).