This is a knockout-validated antibody summary, based on the publication "Proteome Analysis of USP7 Substrates Revealed Its Role in Melanoma Through PI3K/Akt/FOXO and AMPK Pathways", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blotting analysis was performed to determine the expression of USP7 in protein lysates from B16 cells after USP7 gene deletion using the CRISPR/Cas9 editing system (USP7 KO). From [1].

Mouse monoclonal IgG3 κ

Company: Santa Cruz

Antibody: HAUSP

Catalog number: sc-377147

Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 17-53 near the N-terminus of human HAUSP. Recommended for detection of HAUSP of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

WT and USP7-KO B16 cells.

1:400 dilution.

1:20,000 dilution anti-mouse IgG (Abclonal, AS003).