This is a knockout-validated antibody summary, based on the publications "The Tumor Suppressor Hace1 Is a Critical Regulator of TNFR1-Mediated Cell Fate", as cited below [1], and "HACE1 blocks HIF1α accumulation under hypoxia in a RAC1 dependent manner" for western blot knockout validation (figure 2d) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot with HACE1 antibody EPR7962 in Hace1+/+ (left six lanes) and hace1–/– (right six lanes) MEFs. From Figure 1A in [1].

Company: Abcam

Antibody: HACE1

Catalog number: ab133637. The article indicates OriGene TA310566, which is no longer available (except in China). OriGene TA310566 is the same clone as EPR7962, which is sold as Abcam ab133637.

Summary: Suitable for: WBl; Unsuitable for: ICC or IHC-P. Reactive with: Mouse, Rat, Human. The immunogen is a synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to human HACE1 aa 850 to the C-terminus (C terminal).

Western blot

MEFs derived from hace1+/+ and hace1–/– mice were lysed in 1 ml ice-cold NP-40 buffer (10 mM Tris pH 8.0, 150 mM NaCl, and 1% Nonidet P-40) supplemented with protease and phosphatase inhibitor tablets (11873580001 and 04906837001, Roche).