H3.3 antibody | knockout validation | Abnova H00003021-M01
DOI
//dx.doi.org/10.13070/ko.en.7.2150
Date
2017-02-17

This is a knockout-validated antibody summary, based on the publication "Histone H3.3 maintains genome integrity during mammalian development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

H3.3 antibody | knockout validation | Abnova H00003021-M01 figure 1
Figure 1. Western blot of H3.3 protein levels in control and H3f3afl/−; H3f3bfl/−-cKO ES cells with or without TAM. The numbers below are calculated relative protein levels normalized to total H3. From [1].
Antibody information

Mouse monoclonal IgG1 kappa

Company: Abnova

Antibody: H3.3

Catalog number: H00003021-M01

Summary: Mouse monoclonal IgG1 kappa against a full length recombinant H3F3B. Reacts with human by western blot, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Whole histone preparations from control and H3f3afl/−; H3f3bfl/−-cKO embryonic stem cells.

Primary incubation

1:40 dilution.

Clone note

The same clone (2D7-H1) is sold as LifeSpan Biosciences LS-B8442; Abnova H00003021-M01; Epigentek A-4123-100; Sigma-Aldrich WH0003021M1.

References
  1. Jang C, Shibata Y, Starmer J, Yee D, Magnuson T. Histone H3.3 maintains genome integrity during mammalian development. Genes Dev. 2015;29:1377-92 pubmed publisher