This is a knockout-validated antibody summary, based on the publication "The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blots of H2A.X in WT and different H2A.X-KO clones in HCT116 (upper pannel) and HCT15 (lower pannel) cells. Tubulin and actin were used as loading control. From [1].

Antibody: H2A.X

Catalog number: ab20669

Summary: Rabbit polyclonal raised against a synthetic peptide (conjugated to KLH) derived from within amino acid residues 100 to the C-terminus of human H2A.X.

Reacts with mouse, rat, and human proteins and has been used in ChIP, western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry-paraffin.

Company: Abcam

Western blot

HCT116 parental cells (WT) and H2A.X knockout cells (KO) and HCT15 H2A.X KO cells. Cells were washed twice with PBS and directly solubilized in denaturing sample buffer.

1:2,000

horseradish peroxidise-coupled anti-rabbit or anti-mouse IgG antibodies (Amersham, GE Healthcare, Pittsburgh, PA, USA).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.