This is a knockout-validated antibody summary, based on the publication "Loss of glycine receptors containing the α3 subunit compromises auditory nerve activity, but not outer hair cell function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1
Company: Synaptic Systems
Antibody: Glycine receptor
Catalog number: 146 011
Summary: Mouse monoclonal IgG1 against purified rat spinal cord glycine receptor. Reacts with human, rat, mouse, pig, and zebrafish. Suitable for western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry and ELISA.
Immunohistochemistry
Glra3+/+ and Glra3-/- mouse cochlea.
Cy3-conjugated secondary antibody from goat (Jackson ImmunoResearch Laboratories, Westgrove, PA, USA).
Specimens were photographed using an ApoTome microscope (Zeiss, Jena, Germany; objective: C-APOCHROMAT 40x / 1.2 W, numerical aperture: 1.2).
Immunostaining of cochlear cryosections from (a) Glra3+/+ and (b) Glra3-/- mice with the pan-GlyRα antibody mAb4a (red). Cell nuclei were stained with DAPI (blue). Scale bar: 20 µm. Please see Figure 1 in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.