Glutamine-rich 2 antibody | knockout validation | Santa Cruz sc-514279

This is a knockout-validated antibody summary, based on the publication "Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Glutamine-rich 2 antibody | knockout validation | Santa Cruz sc-514279 figure 1
Figure 1. Qrich2 protein levels in the testes of the WT and KO mice. From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Glutamine-rich 2

Catalog number: sc-514279

Summary: Mouse monoclonal IgG1 κ Glutamine-rich 2 antibody against amino acids 1151-1426 mapping within an internal region of human Glutamine-rich 2. Recommended for detection of Glutamine-rich 2 of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

WT and QRICH2-KO mice testes.

Primary incubation

1:200 dilution.

References
  1. Shen Y, Zhang F, Li F, Jiang X, Yang Y, Li X, et al. Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. Nat Commun. 2019;10:433 pubmed publisher