GlcAT-I antibody | knockout validation | Santa Cruz sc-390475

This is a knockout-validated antibody summary, based on the publication "N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GlcAT-I antibody | knockout validation | Santa Cruz sc-390475 figure 1
Figure 1. The efficiency of B3GAT3 gene knockout was determined by western blot analysis with the indicated antibodies. GAPDH served as the loading control. From [1].
Antibody information

Mouse monoclonal IgM κ

Company: Santa Cruz

Antibody: GlcAT-I

Catalog number: sc-390475

Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 323-334 at the C-terminus of human GlcAT-I (B3GAT3). Recommended for detection of GlcAT-I of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Control and B3GAT3-KO HeLa cells.

Secondary incubation

Corresponding secondary HRP antibodies.

Detection

Enhanced chemiluminescent detection reagent (Thermo Fisher Scientific).

References
  1. Song N, Chen L, Ren X, Waterfield N, Yang J, Yang G. N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells. PLoS Pathog. 2021;17:e1009244 pubmed publisher