This is a knockout-validated antibody summary, based on the publication "Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Goat polyclonal
Company: Santa Cruz Biotechnology
Antibody: Geminin
Catalog number: SC-8449
Summary: Goat polyclonal raised against a peptide corresponding to the N-terminal region of mouse geminin. The antibody was affinity purified.
Reacts with mouse, rat and human geminin.
Note: this antibody will be discontinued December 31, 2016.
Immunocytochemistry
Oocytes from wild-type and Gdf9-cre Gmnnfl/fl mice.
Cells fixed with 3.7% paraformaldehyde in PBS for 1 hour then permeabilised with 0.5% Tritin X-100 in PBS for 20 minutes at room temperature.
Overnight with a 1:50 dilution of antibody.
Alexa Fluor conjugated antibody (1:1000).
Zeiss LSM710 laser-scanning confocal microscope.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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