This is a knockout-validated antibody summary, based on the publication "Geminin deletion in mouse oocytes results in impaired embryo development and reduced fertility", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal.
Company: Bioworld
Antibody: Geminin
Catalog number: BS7535
Summary: Rabbit polyclonal raised against full-length recombinant human geminin.
The antiserum was affinity purified against the immunogen.
Reacts with human, rat and mouse geminin.
Supplier recommended for Western blot and IHC.
Western blot
Oocyte lysates from wild-type and Gdf9-cre Gmnnfl/fl mice.
TBS pH 7.4 containing 5% dried milk powder and 0.1% Tween 20.
Primary antibody diluted 1:100 in blocking buffer.
Incubated for 2 hours at 37oC.
HRP-conjugated secondary antibody (1:1000) in blocking buffer for 1 hour at 37oC.
Thermo Super Signal West chemiluminescent substrate.
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
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