This is a knockout-validated antibody summary, based on the publication "ASC filament formation serves as a signal amplification mechanism for inflammasomes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. From Supplementary Figure S8 in [1]. Western blot of lysates of immortalise wild-type and gasdermin-D knockout BMDMs probed with anti-gasdermin antibody. Actin serves as a loading control.

Rabbit polyclonal

Company: SigmaAldrich

Antibody: Gasdermin-D

Catalog number: G7422

Summary: Rabbit polyclonal antibody raised against a synthetic peptide corresponding to residues 126-138 of human gasdermin-D. Supplier recommended for WB and IF. Reacts with human gasdermin-D.

Western blot

Lysates of immortalised wild-type and gasdermin-D knockout bone marrow derived macrophages (BMDMs).

TBS-T containing 5% milk powder.

Anti-gasdermin antibody diluted 1:2000 in blocking buffer.

HRP-conjugated secondary antibody diluted 1:5000 in blocking buffer.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.