Gab2 antibody | knockout validation | Cell Signaling 3239

This is a knockout-validated antibody summary, based on publications "Gab1 mediates PDGF signaling and is essential to oligodendrocyte differentiation and CNS myelination", as cited below [1], and "Gab2 deficiency suppresses high-fat diet-induced obesity by reducing adipose tissue inflammation and increasing brown adipose function in mice" for western blot knockout validation (figure 5b, 5i, 6c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Gab2 antibody | knockout validation | Cell Signaling 3239 figure 1
Figure 1. Western blots of myelin proteins in the cerebral cortex (CTX) and the cerebellum (CB) from WT, Gab2-/- and Gab2+/- mice at P21. From [1].
Antibody information

Company: Cell Signaling

Antibody: Gab2

Catalog number: 3239

Summary: Rabbit monoclonal IgG against recombinant human Gab2 protein. Reacts with human and mouse by western blot.

Validation Method

Western blot

Sample

WT, Gab2+/- and Gab2-/-mice cerebral cortex and the cerebellum.

Primary incubation

1:1,000 dilution.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher Scientific, 31460).

Detection

Enhanced chemiluminescence (Pierce Biotechnology).

References
  1. Zhou L, Shao C, Xie Y, Wang N, Xu S, Luo B, et al. Gab1 mediates PDGF signaling and is essential to oligodendrocyte differentiation and CNS myelination. elife. 2020;9: pubmed publisher
  2. Wang X, Zhao Y, Zhou D, Tian Y, Feng G, Lu Z. Gab2 deficiency suppresses high-fat diet-induced obesity by reducing adipose tissue inflammation and increasing brown adipose function in mice. Cell Death Dis. 2021;12:212 pubmed publisher