This is a knockout-validated antibody summary, based on the publication "Tumour suppressor TRIM33 targets nuclear β-catenin degradation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit polyclonal IgG
Company: Santa Cruz
Antibody: GSK-3
Catalog number: sc-9166
Summary: Rabbit polyclonal IgG against an epitope corresponding to amino acids 345-420 mapping at the C-terminus of human GSK-3β. Recommended for detection of GSK-3β of human, mouse, and rat by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including equine, canine, bovine, porcine and avian.
Western blot
Nuclear extracts from GSK-3β−/− MEFs.
1:1,000 dilution.
Validation has no positive control.
Please see Figure 3a in the article [1].
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
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