GSK-3α antibody | knockout validation | Cell Signaling Technology 9338
DOI
//dx.doi.org/10.13070/ko.en.6.1915
Date
2016-12-12

This is a knockout-validated antibody summary, based on the publication "Fine-Tuning of the RIG-I-Like Receptor/Interferon Regulatory Factor 3-Dependent Antiviral Innate Immune Response by the Glycogen Synthase Kinase 3/β-Catenin Pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit polyclonal

Company: Cell Signaling Technology

Antibody: GSK-3α

Catalog number: 9338

Summary: Rabbit polyclonal antibody raised against a synthetic peptide based on the human GSK-3α sequence.

Supplier recommended for WB.

Peptide affinity-purified.

Reacts with human, mouse rat and monkey GSK-3α. Detects total GSK-3α.

Validation Method

Western blot

Sample

Mouse embryonic fibroblasts from wild-type, GSK-3α knockout and GSK-3α / GSK-3β double knockout animals.

Primary incubation

Anti-GSK-3α antibody.

Secondary incubation

HRP-conjugated secondary antibody (KPL).

Detection

West Lightning ECL kit (Perkin Elmer).

Figure

See Figure 1 from [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Khan K, Dô F, Marineau A, Doyon P, Clément J, Woodgett J, et al. Fine-Tuning of the RIG-I-Like Receptor/Interferon Regulatory Factor 3-Dependent Antiviral Innate Immune Response by the Glycogen Synthase Kinase 3/β-Catenin Pathway. Mol Cell Biol. 2015;35:3029-43 pubmed publisher